Comparison Data of Western Blotting Substrate with Chemi-Lumi One Super and competitors

Chemi-Lumi One Super

  • High sensitivity - detects proteins at Mid-femtogram level with low background
  • Fast - rapid substrate processing of blot


>> for more information on Chemi-Lumi One Super

Comparison Data 1

High Sensitivity and Low Background

Comparison of sensitivity and background using Chemi-Lumi One Super and competitor substrate. To increase sensitivity, the prolongation of exposure time and the application of Signal Enhancer HIKARI treated. The competitor substrate shows high background. The combine Chemi-Lumi One Super and Blocking One (as blocking reagent) or Signal Enhancer HIKARI show lower background than competitor　substrate.

Sample HL-60 whole cell lysate
Lane 1. 0.5 mg
Lane 2. 0.25 mg
Lane 3. 0.125 mg
Blocking Chemi-Lumi One Super: Blocking One (#03953-95), 30 min.
Competitor A: blocking reagent, 60 min.
Primary Antibody mouse anti-b-actin (Santa Cruz: #sc-47778), 1 : 1000
Secondary Antibody goat anti-mouse IgG-HRP (Santa Cruz: #sc-2005), 1 : 10,000
Reaction time Chemi-Lumi One Super: 1 min.
Competitor A: 5min.
Detection LAS-3000 (Sensitivity: High, Expose time: 1min & 10 min.)

Comparison Data 2

High Sensitivity

Chemi-Lumi One Super is much more sensitive than Competitor B Substrate.

Comparison Data 3

Reduce exposure time

Chemi-Lumi One Super can obtain the same sensitivity with much shorter exposure time compared to competitor B.

30 minutes exposure Primary Ab 1 : 3000 Secondary Ab 1 : 10000

Comparison Data 4

Reduce the amount of antibodies

Chemi-Lumi One enables customers to reduce the amount of their precious primary antibodies by three times.

Comparison Data 5

Reduce the amount of antigens

Chemi-Lumi One enables customers to reduce the amount of their antigens by two to four times.

30 minutes exposure Primary Ab 1 : 3000 Secondary Ab 1 : 10000

Chemi-Lumi One L

  • Longer light emission　 - strong light emission for more than 120 minutes
  • Highly sensitive - intense signal with low background
  • Fast - rapid substrate processing of blot


Comparison Data 6

More sensitive and longer duration

Chemi-Lumi One L has more sensitive and longer signal duration than Competitor C.

Ordering Information

Product name Storage Product No. PKG Size
Chemi-Lumi One Super
  Luminol 50 ml, Peroxide 50 ml
  Sufficient substrate for 1,000 cm of blotting membrane		 R 02230-30 1 kit
Chemi-Lumi One L
  Luminol 250 ml, Peroxide 250 ml
  Sufficient substrate for 4,000 cm of blotting membrane		 R 07880-70 1 kit

(Storage) R = Refrigerator

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